Effect of diet and age on arterial stiffening due to atherosclerosis in ApoE-/- mice

Cilla Hernandez, Myriam; Pérez Rontome, Marta; Peña Baquedano, Estefanía; Martínez Barca, Miguel Ángel

doi:10.1007/s10439-015-1486-1

Effect of diet and age on arterial stiffening due to atherosclerosis in ApoE-/- mice

Cilla Hernandez, Myriam ; Pérez Rontome, Marta (Universidad de Zaragoza) ; Peña Baquedano, Estefanía (Universidad de Zaragoza) ; Martínez Barca, Miguel Ángel (Universidad de Zaragoza)

Resumen: This work analyzes the progressive stiffening of the aorta due to atherosclerosis development of both ApoE-/- and C57BL/6J mice fed on a Western (n = 5) and a normal (n = 5) chow diet for the ApoE-/- group and on a normal chow diet (n = 5) for the C57BL/6J group. Sets of 5 animals from the three groups were killed after 10, 20, 30 and 40 weeks on their respective diets (corresponding to 17, 27, 37 and 47 weeks of age). Mechanical properties (inflation test and axial residual stress measurements) and histological properties were compared for both strains, ApoE-/- on the hyper-lipidic diet and both ApoE-/- and C57BL/6J on the normal diet, after the same period and after different periods of diet. The results indicated that the aorta stiffness in the ApoE-/- and C57BL/6J mice under normal diet remained approximately constant irrespective of their age. However, the arterial stiffness in the ApoE-/- on the hyper-lipidic diet increased over time. Statistical differences were found between the group after 10 weeks and the groups after 30 and 40 weeks of a hyper-lipidic diet. Comparing the hyper-lipidic and normal diet mice, statistical differences were also found between both diets in all cases after 40 weeks of diet, frequently after 30 weeks, and in some cases after 20 weeks. The early stages of lesion corresponded to the first 2 weeks of diet. Advanced lesions were found at 30 weeks and, finally, the aorta was completely damaged after 40 weeks of diet. In conclusion, we found substantial changes in the mechanical properties of the aorta walls of the ApoE-/- mice fed with the hyper-lipidic diet compared to the normal chow diet groups for both the ApoE-/- and C57BL/6J groups. These findings could serve as a reference for the study of changes in the arterial wall properties in cases of atherosclerosis.
Idioma: Inglés
DOI: 10.1007/s10439-015-1486-1
Año: 2016
Publicado en: ANNALS OF BIOMEDICAL ENGINEERING 44, 7 (2016), 2202–2217
ISSN: 0090-6964
Financiación: info:eu-repo/grantAgreement/ES/DGA/T88
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2013-44391-P
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PRI-AIBDE-2011-1216
Financiación: info:eu-repo/grantAgreement/ES/UZ/2008-BIO-21
Tipo y forma: Article (PostPrint)
Área (Departamento): Anatomía y Anatomía Patológica Comparadas (Departamento de Anatomía, Embriología y Genética Animal)
Área (Departamento): Mec. de Medios Contínuos y Teor. de Estructuras (Departamento de Ingeniería Mecánica)

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Este artículo se encuentra en las siguientes colecciones:
Articles > Artículos por área > Mec. de Medios Contínuos y Teor. de Estructuras
Articles > Artículos por área > Anatomía y Anatomía Patológica Comparadas

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Effect of diet and age on arterial stiffening due to atherosclerosis in ApoE-/- mice