Estimation of the relative sensitivity of qpcr analysis using pooled samples

Muniesa, Ana; Ferreira González, Chelo; Fuertes, Héctor; Nalaihel, Nabil; de Blas, Ignacio

doi:10.1371/journal.pone.0093491

Estimation of the relative sensitivity of qpcr analysis using pooled samples

Muniesa, Ana ; Ferreira González, Chelo (Universidad de Zaragoza) ; Fuertes, Héctor ; Nalaihel, Nabil ; de Blas, Ignacio

Resumen: The high sensitivity of qPCR makes it a desirable diagnostic method in epidemiological surveillance programs. However, due to high costs, the use of pooling has been suggested. In this paper, an algorithm based on the Montecarlo method has been designed and implemented. The algorithm had been tested in many different situations, and finally it was validated with a real dataset. Moreover, based on the results obtained and depending on pooling conditions, a drastic decrease of sensitivity is observed.
Idioma: Inglés
DOI: 10.1371/journal.pone.0093491
Año: 2014
Publicado en: PLoS One 9, 4 (2014), e93491 [5 pp]
ISSN: 1932-6203
Factor impacto JCR: 3.234 (2014)
Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 8 / 56 = 0.143 (2014) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/MICINN/MTM2010-21037
Tipo y forma: Article (Published version)
Área (Departamento): Matemática Aplicada (Departamento de Matemática Aplicada)
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Estimation of the relative sensitivity of qpcr analysis using pooled samples