Morphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men
Santolaria Blasco, Pilar (Universidad de Zaragoza) ; Soler, C. ; Recreo Tome, María Pilar ; Carretero López-Tello, María Teresa (Universidad de Zaragoza) ; Bono, A. ; Berné, J. ; Yániz Pérez De Albéniz, Jesús Luis (Universidad de Zaragoza)
Resumen: This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations (slow with low ALH 35.6% of all motile spermatozoa], with circular trajectories 32.0%], and rapid with high ALH 32.4%]), and three morphometric subpopulations (large-round 33.9% of all spermatozoa], elongated 32.0%], and small 34.10%]). The distribution of kinematic sperm subpopulations was different among ejaculate fractions (P < 0.001), with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions (P < 0.001), with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.
Idioma: Inglés
DOI: 10.4103/1008-682X.186874
Año: 2016
Publicado en: ASIAN JOURNAL OF ANDROLOGY 18, 6 (2016), 831-834
ISSN: 1008-682X
Financiación: info:eu-repo/grantAgreement/ES/DGA/A40
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P
Tipo y forma: Article (Published version)
Área (Departamento): Producción Animal (Departamento de Producción Animal y Ciencia de los Alimentos)
Área (Departamento): Anatomía y Embriología Humana (Departamento de Anatomía e Histología Humanas)
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Exportado de SIDERAL (2016-12-13-10:03:39)
Idioma: Inglés
DOI: 10.4103/1008-682X.186874
Año: 2016
Publicado en: ASIAN JOURNAL OF ANDROLOGY 18, 6 (2016), 831-834
ISSN: 1008-682X
Financiación: info:eu-repo/grantAgreement/ES/DGA/A40
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P
Tipo y forma: Article (Published version)
Área (Departamento): Producción Animal (Departamento de Producción Animal y Ciencia de los Alimentos)
Área (Departamento): Anatomía y Embriología Humana (Departamento de Anatomía e Histología Humanas)
0
Exportado de SIDERAL (2016-12-13-10:03:39)
Este artículo se encuentra en las siguientes colecciones:
articulos > articulos-por-area > anatomia_y_embriologia_humana
articulos > articulos-por-area > produccion_animal
Notice créée le 2016-12-13, modifiée le 2016-12-13
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