Direct infussion Electrospray Mass Spectrometry as a new non-invasive tool for serum metabolomics in induced-stress subjects
Lorenzo-Tejedor, Mónica ; de la Cámara Izquierdo, Concepción (Universidad de Zaragoza) ; López Antón, Raul (Universidad de Zaragoza) ; Bailón Luesma, Raquel (Universidad de Zaragoza) ; Aguiló Llobet, Jordi ; Bernal Ruiz, María Luisa (Universidad de Zaragoza)
Resumen: Background and Objectives: Nanotechnology is becoming a tool for the study of changes in the metabolome of patients in different states of disease. Analytical techniques such as Electrospray Mass Spectrometry, allow to find biomarkers by determi- nation of metabolites. Nowadays, there is not an objective analytical approach for diagno- sis of stress. Thus, the objectives of this pilot work are:
– Describing the development of a fast, direct and non-invasive analytical protocol, ap- plied for the first time, to study the metabolomic profile of patient s different states through a disease.
– Testing the protocol in a pilot sample with non-stressed and stress-induced subjects.
Methods: High resolution direct infusion electrospray mass spectrometry has been used to analyse the metabolome of blood samples (0.3 ml) from six subject s.
Results: Data prove a clear discrimination between non-stressed and stressed states in the metabolome. Data showed different predominant metabolites in both states. Results allow objective characterization of the state of the patient.
Conclusions: Although this is a pilot study, the method was successful in discriminating different metabolites in non-stressed and stress-induced subjects.
Idioma: Inglés
Año: 2015
Publicado en: EUROPEAN JOURNAL OF PSYCHIATRY 29, 4 (2015), 259-275
ISSN: 0213-6163
Factor impacto JCR: 0.711 (2015)
Categ. JCR: PSYCHIATRY rank: 170 / 197 = 0.863 (2015) - Q4 - T3
Tipo y forma: Article (Published version)
Área (Departamento): Psiquiatría (Departamento de Medicina, Psiquiatría y Dermatología)
Área (Departamento): Teoría de la Señal y Comunicaciones (Departamento de Ingeniería Electrónica y Comunicaciones)
Área (Departamento): Farmacología (Departamento de Farmacología y Fisiología)
Área (Departamento): Psicología Básica (Departamento de Psicología y Sociología)
0
Exportado de SIDERAL (2016-06-27-10:22:26)
– Describing the development of a fast, direct and non-invasive analytical protocol, ap- plied for the first time, to study the metabolomic profile of patient s different states through a disease.
– Testing the protocol in a pilot sample with non-stressed and stress-induced subjects.
Methods: High resolution direct infusion electrospray mass spectrometry has been used to analyse the metabolome of blood samples (0.3 ml) from six subject s.
Results: Data prove a clear discrimination between non-stressed and stressed states in the metabolome. Data showed different predominant metabolites in both states. Results allow objective characterization of the state of the patient.
Conclusions: Although this is a pilot study, the method was successful in discriminating different metabolites in non-stressed and stress-induced subjects.
Idioma: Inglés
Año: 2015
Publicado en: EUROPEAN JOURNAL OF PSYCHIATRY 29, 4 (2015), 259-275
ISSN: 0213-6163
Factor impacto JCR: 0.711 (2015)
Categ. JCR: PSYCHIATRY rank: 170 / 197 = 0.863 (2015) - Q4 - T3
Tipo y forma: Article (Published version)
Área (Departamento): Psiquiatría (Departamento de Medicina, Psiquiatría y Dermatología)
Área (Departamento): Teoría de la Señal y Comunicaciones (Departamento de Ingeniería Electrónica y Comunicaciones)
Área (Departamento): Farmacología (Departamento de Farmacología y Fisiología)
Área (Departamento): Psicología Básica (Departamento de Psicología y Sociología)
0
Exportado de SIDERAL (2016-06-27-10:22:26)
Este artículo se encuentra en las siguientes colecciones:
articulos > articulos-por-area > teoria_de_la_senal_y_comunicaciones
articulos > articulos-por-area > psicologia_basica
articulos > articulos-por-area > farmacologia
articulos > articulos-por-area > psiquiatria
Notice créée le 2016-05-06, modifiée le 2016-10-06
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