Evaluation of lasting effects of heat stress on sperm profile and oxidative status of ram semen and epididymal sperm
Hamilton, T. R. D. S. ; Mendes, C. M. ; Castro, L. S. D. ; Assis, P. M. D. ; Siqueira, A. F. P. ; Delgado, J. D. C. ; Goissis, M. D. ; Muiño Blanco, María Teresa (Universidad de Zaragoza) ; Cebrián Pérez, José Álvaro (Universidad de Zaragoza) ; Nichi, M. ; Visintin, J. A. ; Assumpção, M. E. O. D.
Resumen: Higher temperatures lead to an increase of testicular metabolism that results in spermatic damage. Oxidative stress is the main factor responsible for testicular damage caused by heat stress. The aim of this study was to evaluate lasting effects of heat stress on ejaculated sperm and immediate or long-term effects of heat stress on epididymal sperm. We observed decrease in motility and mass motility of ejaculated sperm, as well as an increase in the percentages of sperm showing major and minor defects, damaged plasma and acrosome membranes, and a decrease in the percentage of sperm with high mitochondrial membrane potential in the treated group until one spermatic cycle. An increased enzymatic activity of glutathione peroxidase and an increase of stressed cells were observed in ejaculated sperm of the treated group. A decrease in the percentage of epididymal sperm with high mitochondrial membrane potential was observed in the treated group. However, when comparing immediate and long-term effects, we observed an increase in the percentage of sperm with low mitochondrial membrane potential. In conclusion, testicular heat stress induced oxidative stress that led to rescuable alterations after one spermatic cycle in ejaculated sperm and also after 30 days in epididymal sperm.
Idioma: Inglés
DOI: 10.1155/2016/1687657
Año: 2016
Publicado en: Oxidative Medicine and Cellular Longevity 2016 (2016), 1687657 [12pp]
ISSN: 1942-0900
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2013-43328-P
Tipo y forma: Article (Published version)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)
Área (Departamento): Zoología (Departamento de Bioquímica y Biología Molecular y Celular)
0
Exportado de SIDERAL (2016-03-10-16:28:32)
Idioma: Inglés
DOI: 10.1155/2016/1687657
Año: 2016
Publicado en: Oxidative Medicine and Cellular Longevity 2016 (2016), 1687657 [12pp]
ISSN: 1942-0900
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2013-43328-P
Tipo y forma: Article (Published version)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)
Área (Departamento): Zoología (Departamento de Bioquímica y Biología Molecular y Celular)
0
Exportado de SIDERAL (2016-03-10-16:28:32)
Este artículo se encuentra en las siguientes colecciones:
articulos > articulos-por-area > bioquimica_y_biologia_molecular
articulos > articulos-por-area > zoologia
Notice créée le 2016-03-03, modifiée le 2016-10-06
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