gamma-Lindane increases microcystin synthesis in microcystis aeruginosa PCC7806
Ceballos Laita, Laura ; Calvo Beguería, Laura María ; Lahoz, J. ; Bes Fustero, María Teresa (Universidad de Zaragoza) ; Fillat Castejón, María Francisca (Universidad de Zaragoza) ; Peleato Sánchez, María Luisa (Universidad de Zaragoza)
Resumen: HCH factories, and the waste dumpsites associated to its production, have become a global environmental concern, and their runoff could pollute ground and surface waters with high levels of the pollutant. In this study, the influence of lindane (¿-HCH) on microcystin production has been investigated in Microcystis aeruginosa PCC7806. This toxic cyanobacterium is highly tolerant to ¿-lindane (20 mg/L), and produces more toxin (microcystin) in the presence of the pollutant. Microcystis degrades ¿-lindane and presence of ¿-lindane induces genes involved in its own degradation (nirA). RT-PCRsq has been used to monitor changes in levels of transcripts encoded by the mcy operon (mcyD, mcyH and mcyJ), responsible for the microcystin synthesis machinery, as well as other genes involved in its transcriptional regulation, such as ntcA and fur family members. The presence of lindane in the culture media induces mcyD expression, as well as ntcA gene transcription, while other genes, such as mcyH, (putative ABC transporter), are downregulated. The amount of microcystin found in the cells and the culture media is higher when M. aeruginosa is treated with ¿-lindane than in control cells. The results\ suggest that in a lindane polluted environment, Microcystis toxic strains may enhance their microcystin synthesis.
Idioma: Inglés
DOI: 10.3390/md13095666
Año: 2015
Publicado en: Marine Drugs 13, 9 (2015), 5666-5680
ISSN: 1660-3397
Factor impacto JCR: 3.345 (2015)
Categ. JCR: CHEMISTRY, MEDICINAL rank: 13 / 58 = 0.224 (2015) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458
Tipo y forma: Article (Published version)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)
Área (Departamento): Fisiología Vegetal (Departamento de Bioquímica y Biología Molecular y Celular)
0
Exportado de SIDERAL (2016-06-27-09:48:06)
Idioma: Inglés
DOI: 10.3390/md13095666
Año: 2015
Publicado en: Marine Drugs 13, 9 (2015), 5666-5680
ISSN: 1660-3397
Factor impacto JCR: 3.345 (2015)
Categ. JCR: CHEMISTRY, MEDICINAL rank: 13 / 58 = 0.224 (2015) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458
Tipo y forma: Article (Published version)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)
Área (Departamento): Fisiología Vegetal (Departamento de Bioquímica y Biología Molecular y Celular)
0
Exportado de SIDERAL (2016-06-27-09:48:06)
Este artículo se encuentra en las siguientes colecciones:
articulos > articulos-por-area > bioquimica_y_biologia_molecular
articulos > articulos-por-area > fisiologia_vegetal
Notice créée le 2015-11-07, modifiée le 2016-10-06
Liens externes:
Versión publicada
Versión publicada
Versión publicada
Versión publicada