Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
Gómez, Álex ; Glaria, Idoia ; Moncayola, Irati ; Echeverría, Irache ; Rodríguez-Largo, Ana ; de Blas, Ignacio (Universidad de Zaragoza) ; Pérez, Estela (Universidad de Zaragoza) ; Pérez, Marta (Universidad de Zaragoza) ; Villanueva-Saz, Sergio (Universidad de Zaragoza) ; Lee, Benhur ; Diego, Alicia de ; Miguel, Ricardo de ; Luján, Lluís (Universidad de Zaragoza) ; Reina, Ramsés
Resumen: Small ruminant lentiviruses (SRLV) are responsible for significant economic losses in sheep and goat farming; however, effective vaccination strategies remain unavailable. This study evaluated the immunogenicity, safety, and protective efficacy of a recombinant Sendai virus vector (SeV) expressing SRLV gag-P25 (rSeV-GFP-P25) in lambs. Twenty-one SRLV-negative lambs were divided into three groups and inoculated intranasally thrice with culture medium (group 1); SeV-GFP (group 2) or rSeV-GFP-P25 (group 3). Lambs were challenged with homologous SRLV at 16 weeks post-first immunization. Clinical and hematological parameters, antibody responses, SRLV viral loads in peripheral blood mononuclear cells (PBMCs) and target tissues, histopathological and histomorphometric analyses, assisted with artificial intelligence, of interstitial pneumonia were assessed. No clinicopathological alterations were observed, except for a transient temperature increase in group 3 post-first immunization. Group 2 showed mild SeV-neutralizing antibodies, while rSeV-GFP-P25 (group 3) induced negligible SRLV-specific antibody responses. Group 3 exhibited higher SRLV DNA copies in PBMCs but lower in most SRLV target tissues compared to control groups, with no SRLV DNA detected in spleen and bone marrow. Histomorphometry revealed reduced alveolar septal thickening in group 3, indicating partial protection against early SRLV-associated interstitial pneumonia. These results warrant further investigation into cellular immunity and long-term protection.
Idioma: Inglés
DOI: 10.1080/01652176.2025.2556492
Año: 2025
Publicado en: VETERINARY QUARTERLY 45, 1 (2025), 16
ISSN: 0165-2176
Financiación: info:eu-repo/grantAgreement/ES/MICINN/RTI2018-096172-B-C33
Tipo y forma: Article (Published version)
Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Anatom.Anatom.Patológ.Com (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
0
Exportado de SIDERAL (2025-10-17-14:07:38)
Idioma: Inglés
DOI: 10.1080/01652176.2025.2556492
Año: 2025
Publicado en: VETERINARY QUARTERLY 45, 1 (2025), 16
ISSN: 0165-2176
Financiación: info:eu-repo/grantAgreement/ES/MICINN/RTI2018-096172-B-C33
Tipo y forma: Article (Published version)
Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Anatom.Anatom.Patológ.Com (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
0
Exportado de SIDERAL (2025-10-17-14:07:38)
Este artículo se encuentra en las siguientes colecciones:
articulos > articulos-por-area > anatomia_y_anatomia_patologica_comparadas
articulos > articulos-por-area > medicina_y_cirugia_animal
articulos > articulos-por-area > quimica_analitica
articulos > articulos-por-area > sanidad_animal
Notice créée le 2025-10-17, modifiée le 2025-10-17
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