Influence of green manuring on soil properties, abundance and expression of key denitrification genes in a greenhouse Anthrosol
Hernández Maqueda, R. ; Ballesteros, I. ; Aguirre, A. J. (Universidad de Zaragoza) ; Meca, D. ; Linacero, R. ; Moral, F. del
Resumen: Intercropping with green manure is a valuable agronomic practice for promoting sustainability agricultural systems. The aim of this study was to evaluate the effects of green manure (GM) – based on a mixture of vetch (Vicia sativa L.), forage turnip (Brassica rapa L. subsp. rapa), and oats (Avena sativa L.) – on the physicochemical properties of a greenhouse Anthrosol and its impact on the abundance and expression of denitrifying genes (nirK, nirS, and nosZ (clade I and II)) using real-time PCR. The cultivated crop throughout the three-year experiment was eggplant (Solanum melongena L. subs. Telma) fertilized with manure and crop residues from the previous growing season. A linear mixed model (LMM) was used to assess the effect of GM on both the physicochemical variables and the target-gene and transcript copy number in the soil. After three years of GM coverage, moderate changes in soil physicochemical properties were observed, with the exception of a significant decrease in soil temperature (<6 %) and ammonium ion concentration (<14 %) with GM application. Gene copy numbers remained largely unchanged between treatments, however transcripts levels decreased for all target genes under GM, with particularly pronounced reduction for nirK (p < 0.0001) and nosZ II (p < 0.05), suggesting partial suppression of gene transcription within the denitrification pathway. In addition, potassium (K+) and soil moisture were found to correlate with DNA and RNA abundances, indicating a complex interaction between salinity and soil moisture in the regulation of the denitrification process.
Idioma: Inglés
DOI: 10.1016/j.apsoil.2025.106216
Año: 2025
Publicado en: APPLIED SOIL ECOLOGY 212 (2025), 106216 [9 p.]
ISSN: 0929-1393
Tipo y forma: Article (Published version)
Área (Departamento): Área Proyectos de Ingeniería (Dpto. Ingeniería Diseño Fabri.)
0
Exportado de SIDERAL (2025-10-17-14:33:28)
Idioma: Inglés
DOI: 10.1016/j.apsoil.2025.106216
Año: 2025
Publicado en: APPLIED SOIL ECOLOGY 212 (2025), 106216 [9 p.]
ISSN: 0929-1393
Tipo y forma: Article (Published version)
Área (Departamento): Área Proyectos de Ingeniería (Dpto. Ingeniería Diseño Fabri.)
0
Exportado de SIDERAL (2025-10-17-14:33:28)
Este artículo se encuentra en las siguientes colecciones:
articulos > articulos-por-area > proyectos_de_ingenieria
Notice créée le 2025-06-12, modifiée le 2025-10-17
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