000057793 001__ 57793
000057793 005__ 20161213122925.0
000057793 0247_ $$2doi$$a10.1080/10255842.2016.1198784
000057793 0248_ $$2sideral$$a97099
000057793 037__ $$aART-2017-97099
000057793 041__ $$aeng
000057793 100__ $$aRibeiro, F. O.
000057793 245__ $$aComputational model of mesenchymal migration in 3D under chemotaxis
000057793 260__ $$c2017
000057793 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057793 5203_ $$aCell chemotaxis is an important characteristic of cellular migration, which takes part in crucial aspects of life and development. In this work, we propose a novel in silico model of mesenchymal 3D migration with competing protrusions under a chemotactic gradient. Based on recent experimental observations, we identify three main stages that can regulate mesenchymal chemotaxis: chemosensing, dendritic protrusion dynamics and cell–matrix interactions. Therefore, each of these features is considered as a different module of the main regulatory computational algorithm. The numerical model was particularized for the case of fibroblast chemotaxis under a PDGF-bb gradient. Fibroblasts migration was simulated embedded in two different 3D matrices – collagen and fibrin – and under several PDGF-bb concentrations. Validation of the model results was provided through qualitative and quantitative comparison with in vitro studies. Our numerical predictions of cell trajectories and speeds were within the measured in vitro ranges in both collagen and fibrin matrices. Although in fibrin, the migration speed of fibroblasts is very low, because fibrin is a stiffer and more entangling matrix. Testing PDGF-bb concentrations, we noticed that an increment of this factor produces a speed increment. At 1 ng mL-1 a speed peak is reached after which the migration speed diminishes again. Moreover, we observed that fibrin exerts a dampening behavior on migration, significantly affecting the migration efficiency.
000057793 536__ $$9info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2015-64221-C2-1-R
000057793 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000057793 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057793 700__ $$0(orcid)0000-0002-1878-8997$$aGómez Benito, María José$$uUniversidad de Zaragoza
000057793 700__ $$aFolgado, J.
000057793 700__ $$aFernandes, P. R.
000057793 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía Aznar, José Manuel$$uUniversidad de Zaragoza
000057793 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDepartamento de Ingeniería Mecánica$$cMec. de Medios Contínuos y Teor. de Estructuras
000057793 773__ $$g20, 1 (2017), 59-74$$pComput. methods biomech. biomed. eng.$$tCOMPUTER METHODS IN BIOMECHANICS AND BIOMEDICAL ENGINEERING$$x1025-5842
000057793 8564_ $$s3250013$$uhttp://zaguan.unizar.es/record/57793/files/texto_completo.pdf$$yVersión publicada
000057793 8564_ $$s114161$$uhttp://zaguan.unizar.es/record/57793/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057793 909CO $$ooai:zaguan.unizar.es:57793$$particulos$$pdriver
000057793 951__ $$a2016-12-13-10:03:30
000057793 980__ $$aARTICLE