000057773 001__ 57773
000057773 005__ 20161219102840.0
000057773 0247_ $$2doi$$a10.1038/srep36086
000057773 0248_ $$2sideral$$a96860
000057773 037__ $$aART-2016-96860
000057773 041__ $$aeng
000057773 100__ $$0(orcid)0000-0002-9414-1845$$aAyuso Domínguez, José María
000057773 245__ $$aDevelopment and characterization of a microfluidic model of the tumour microenvironment
000057773 260__ $$c2016
000057773 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057773 5203_ $$aThe physical microenvironment of tumours is characterized by heterotypic cell interactions and physiological gradients of nutrients, waste products and oxygen. This tumour microenvironment has a major impact on the biology of cancer cells and their response to chemotherapeutic agents. Despite this, most in vitro cancer research still relies primarily on cells grown in 2D and in isolation in nutrient- and oxygen-rich conditions. Here, a microfluidic device is presented that is easy to use and enables modelling and study of the tumour microenvironment in real-time. The versatility of this microfluidic platform allows for different aspects of the microenvironment to be monitored and dissected. This is exemplified here by real-time profiling of oxygen and glucose concentrations inside the device as well as effects on cell proliferation and growth, ROS generation and apoptosis. Heterotypic cell interactions were also studied. The device provides a live ‘window’ into the microenvironment and could be used to study cancer cells for which it is difficult to generate tumour spheroids. Another major application of the device is the study of effects of the microenvironment on cellular drug responses. Some data is presented for this indicating the device’s potential to enable more physiological in vitro drug screening.
000057773 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/MTM2012-31073$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2011-28262-C04-01$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2015-69967-C3-1
000057773 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057773 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057773 700__ $$0(orcid)0000-0003-3660-8651$$aVirumbrales Muñoz, Maria$$uUniversidad de Zaragoza
000057773 700__ $$aLacueva, A.
000057773 700__ $$aLanuza, P. M.
000057773 700__ $$aCheca-Chavarria, E.
000057773 700__ $$aBotella, P.
000057773 700__ $$aFernández, E.
000057773 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré Castellano, Manuel$$uUniversidad de Zaragoza
000057773 700__ $$aAllison, S. J.
000057773 700__ $$aPhillips, R. M.
000057773 700__ $$0(orcid)0000-0003-0154-0730$$aPardo Jimeno, Julián$$uUniversidad de Zaragoza
000057773 700__ $$0(orcid)0000-0001-5376-4440$$aFernández Ledesma, Luis José$$uUniversidad de Zaragoza
000057773 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa Garrido, Ignacio$$uUniversidad de Zaragoza
000057773 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDepartamento de Anatomía e Histología Humanas$$cHistología
000057773 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDepartamento de Microbiología, Medicina Preventiva y Salud Pública$$cInmunología
000057773 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDepartamento de Bioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular
000057773 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDepartamento de Ingeniería Mecánica$$cMec. de Medios Contínuos y Teor. de Estructuras
000057773 773__ $$g6, 36086 (2016), [16 pp.]$$pSci. rep.$$tSCIENTIFIC REPORTS$$x2045-2322
000057773 8564_ $$s3800383$$uhttp://zaguan.unizar.es/record/57773/files/texto_completo.pdf$$yVersión publicada
000057773 8564_ $$s116626$$uhttp://zaguan.unizar.es/record/57773/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057773 909CO $$ooai:zaguan.unizar.es:57773$$particulos$$pdriver
000057773 951__ $$a2016-12-19-10:13:47
000057773 980__ $$aARTICLE