000056738 001__ 56738
000056738 005__ 20161006102719.0
000056738 0247_ $$2doi$$a10.1371/journal.pone.0159136
000056738 0248_ $$2sideral$$a96002
000056738 037__ $$aART-2016-96002
000056738 041__ $$aeng
000056738 100__ $$aJiménez, S.
000056738 245__ $$aRosa canina extracts have antiproliferative and antioxidant effects on caco-2 human colon cancer
000056738 260__ $$c2016
000056738 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056738 5203_ $$aThe in vitro antiproliferative and antioxidant effects of different fractions of Rosa canina hips on human colon cancer cell lines (Caco-2) was studied. The compounds tested were total extract (fraction 1), vitamin C (fraction 2), neutral polyphenols (fraction 3) and acidic polyphenols (fraction 4). All the extracts showed high cytotoxicity after 72 h, both low and high concentrations. The flow cytometric analysis revealed that all the fractions produce disturbances in the cell cycle resulting in a concomitant cell death by an apoptotic pathway. Changes in the redox status of Caco-2 cells in response to Rosa canina hips were determined. Cells were exposed to hydrogen peroxide in presence of plant fractions and the production of Reactive Oxygen Species (ROS) was significantly decreased. Therefore, our data demonstrate that rosehip extracts are a powerful antioxidant that produces an antiproliferative effect in Caco-2 cells. Therefore, these results predict a promising future for Rosa canina as a therapeutic agent. Thus, this natural plant could be an effective component of functional foods addressed towards colorectal carcinoma.
000056738 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/SAF2013-41651
000056738 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000056738 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056738 700__ $$aGascón Mesa, Sonia
000056738 700__ $$aLuquin Martínez, María Asunción
000056738 700__ $$0(orcid)0000-0002-5801-3352$$aLaguna Castrillo, Mariano
000056738 700__ $$aAncin-Azpilicueta, C.
000056738 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez Yoldi, María Jesús$$uUniversidad de Zaragoza
000056738 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDepartamento de Farmacología y Fisiología$$cFisiología
000056738 773__ $$g11, 7 (2016), 0159136 [14p.]$$pPLoS One$$tPLoS One$$x1932-6203
000056738 8564_ $$s790748$$uhttp://zaguan.unizar.es/record/56738/files/texto_completo.pdf$$yVersión publicada
000056738 8564_ $$s104979$$uhttp://zaguan.unizar.es/record/56738/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056738 909CO $$ooai:zaguan.unizar.es:56738$$particulos$$pdriver
000056738 951__ $$a2016-09-26-11:00:10
000056738 980__ $$aARTICLE