000056180 001__ 56180
000056180 005__ 20161006102712.0
000056180 0247_ $$2doi$$a10.1371/journal.pone.0154268
000056180 0248_ $$2sideral$$a95330
000056180 037__ $$aART-2016-95330
000056180 041__ $$aeng
000056180 100__ $$aPérez-Palacios, R.
000056180 245__ $$aIn vivo chromatin targets of the transcription factor Yin Yang 2 in trophoblast stem cells
000056180 260__ $$c2016
000056180 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056180 5203_ $$aBackground: Yin Yang 2 (YY2) is a zinc finger protein closely related to the well-characterized Yin Yang 1 (YY1). YY1 is a DNA-binding transcription factor, with defined functions in multiple developmental processes, such as implantation, cell differentiation, X inactivation, imprinting and organogenesis. Yy2 has been treated as a largely immaterial duplication of Yy1, as they share high homology in the Zinc Finger-region and similar if not identical in vitro binding sites. In contrast to these similarities, gene expression alterations in HeLa cells with attenuated levels of either Yy1 or Yy2 were to some extent gene-specific. Moreover, the chromatin binding sites for YY2, except for its association with transposable retroviral elements (RE) and Endogenous Retroviral Elements (ERVs), remain to be identified. As a first step towards defining potential Yy2 functions matching or complementary to Yy1, we considered in vivo DNA binding sites of YY2 in trophoblast stem (TS) cells. Results: We report the presence of YY2 protein in mouse-derived embryonic stem (ES) and TS cell lines. Following up on our previous report on ERV binding by YY2 in TS cells, we investigated the tissue-specificity of REX1 and YY2 binding and confirm binding to RE/ERV targets in both ES cells and TS cells. Because of the higher levels of expression, we chose TS cells to understand the role of Yy2 in gene and chromatin regulation. We used in vivo YY2 association as a measure to identify potential target genes. Sequencing of chromatin obtained in chromatin-immunoprecipitation (ChIP) assays carried out with aYY2 serum allowed us to identify a limited number of chromatin targets for YY2. Some putative binding sites were validated in regular ChIP assays and gene expression of genes nearby was altered in the absence of Yy2. Conclusions: YY2 binding to ERVs is not confined to TS cells. In vivo binding sites share the presence of a consensus binding motif. Selected sites were uniquely bound by YY2 as opposed to YY1, suggesting that YY2 exerts unique contributions to gene regulation. YY2 binding was not generally associated with gene promoters. However, several YY2 binding sites are linked to long noncoding RNA (lncRNA) genes and we show that the expression levels of a few of those are Yy2-dependent.
000056180 536__ $$9info:eu-repo/grantAgreement/ES/DGA/PI110-09$$9info:eu-repo/grantAgreement/ES/FIS/PI07119
000056180 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000056180 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056180 700__ $$aMacías-Redondo, S.
000056180 700__ $$0(orcid)0000-0002-0606-0763$$aCliment Aroz, Maria$$uUniversidad de Zaragoza
000056180 700__ $$0(orcid)0000-0002-5462-907X$$aContreras Moreira, Bruno$$uUniversidad de Zaragoza
000056180 700__ $$0(orcid)0000-0003-2014-2869$$aMuniesa Lorda, Pedro$$uUniversidad de Zaragoza
000056180 700__ $$aSchoorlemmer , Jon
000056180 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDepartamento de Bioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular
000056180 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDepartamento de Anatomía, Embriología y Genética Animal$$cAnatomía y Anatomía Patológica Comparadas
000056180 773__ $$g11, 5 (2016), e0154268 [21pp]$$pPLoS One$$tPLoS One$$x1932-6203
000056180 8564_ $$s1924233$$uhttp://zaguan.unizar.es/record/56180/files/texto_completo.pdf$$yVersión publicada
000056180 8564_ $$s103364$$uhttp://zaguan.unizar.es/record/56180/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056180 909CO $$ooai:zaguan.unizar.es:56180$$particulos$$pdriver
000056180 951__ $$a2016-07-14-10:18:52
000056180 980__ $$aARTICLE