000056146 001__ 56146
000056146 005__ 20161006102711.0
000056146 0247_ $$2doi$$a10.1016/j.bbrep.2016.06.007
000056146 0248_ $$2sideral$$a95248
000056146 037__ $$aART-2016-95248
000056146 041__ $$aeng
000056146 100__ $$aEscós, M.
000056146 245__ $$aKinetic and functional properties of human mitochondrial phosphoenolpyruvate carboxykinase
000056146 260__ $$c2016
000056146 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056146 5203_ $$aThe cytosolic form of phosphoenolpyruvate carboxykinase (PCK1) plays a regulatory role in gluconeogenesis and glyceroneogenesis. The role of the mitochondrial isoform (PCK2) remains unclear. We report the partial purification and kinetic and functional characterization of human PCK2. Kinetic properties of the enzyme are very similar to those of the cytosolic enzyme. PCK2 has an absolute requirement for Mn2+ ions for activity; Mg2+ ions reduce the Km for Mn2+ by about 60 fold. Its specificity constant is 100 fold larger for oxaloacetate than for phosphoenolpyruvate suggesting that oxaloacetate phosphorylation is the favored reaction in vivo. The enzyme possesses weak pyruvate kinase-like activity (kcat=2.7 s-1). When overexpressed in HEK293T cells it enhances strongly glucose and lipid production showing that it can play, as the cytosolic isoenzyme, an active role in glyceroneogenesis and gluconeogenesis.
000056146 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A51$$9info:eu-repo/grantAgreement/ES/MEC/AGL2015–66177-R$$9info:eu-repo/grantAgreement/ES/UZ/2015- BIO-01
000056146 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000056146 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056146 700__ $$0(orcid)0000-0002-6794-3535$$aLatorre Muro, Pedro$$uUniversidad de Zaragoza
000056146 700__ $$aHidalgo, J.
000056146 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado Guerrero, Ramon$$uUniversidad de Zaragoza
000056146 700__ $$0(orcid)0000-0003-0062-1029$$aCarrodeguas Villar, José Alberto$$uUniversidad de Zaragoza
000056146 700__ $$0(orcid)0000-0001-5992-2913$$aLópez Buesa, Pascual Luis$$uUniversidad de Zaragoza
000056146 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDepartamento de Bioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular
000056146 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDepartamento de Producción Animal y Ciencia de los Alimentos$$cTecnología de Alimentos
000056146 773__ $$g7 (2016), 124-129$$tBiochemistry and Biophysics Reports$$x2405-5808
000056146 8564_ $$s649502$$uhttp://zaguan.unizar.es/record/56146/files/texto_completo.pdf$$yVersión publicada
000056146 8564_ $$s109613$$uhttp://zaguan.unizar.es/record/56146/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056146 909CO $$ooai:zaguan.unizar.es:56146$$particulos$$pdriver
000056146 951__ $$a2016-07-12-14:49:32
000056146 980__ $$aARTICLE