000036758 001__ 36758
000036758 005__ 20161006102655.0
000036758 0247_ $$2doi$$a10.1371/journal.pone.0139515
000036758 0248_ $$2sideral$$a93128
000036758 037__ $$aART-2015-93128
000036758 041__ $$aeng
000036758 100__ $$0(orcid)0000-0002-9414-1845$$aAyuso Domínguez, José María$$uUniversidad de Zaragoza
000036758 245__ $$aStudy of the chemotactic response of multicellular spheroids in a microfluidic device
000036758 260__ $$c2015
000036758 5060_ $$aAccess copy available to the general public$$fUnrestricted
000036758 5203_ $$aWe report the first application of a microfluidic device to observe chemotactic migration in multicellular spheroids. A microfluidic device was designed comprising a central microchamber and two lateral channels through which reagents can be introduced. Multicellular spheroids were embedded in collagen and introduced to the microchamber. A gradient of fetal bovine serum (FBS) was established across the central chamber by addition of growth media containing serum into one of the lateral channels. We observe that spheroids of oral squamous carcinoma cells OSC-19 invade collectively in the direction of the gradient of FBS. This invasion is more directional and aggressive than that observed for individual cells in the same experimental setup. In contrast to spheroids of OSC-19, U87-MG multicellular spheroids migrate as individual cells. A study of the exposure of spheroids to the chemoattractant shows that the rate of diffusion into the spheroid is slow and thus, the chemoattractant wave engulfs the spheroid before diffusing through it. Copyright:
000036758 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/DPI2011-28262-C04-01
000036758 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000036758 590__ $$a3.057$$b2015
000036758 591__ $$aMULTIDISCIPLINARY SCIENCES$$b11 / 60 = 0.183$$c2015$$dQ1$$eT1
000036758 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000036758 700__ $$aBasheer, H. A.
000036758 700__ $$aMonge, R.
000036758 700__ $$aSánchez-Álvarez, P.
000036758 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré Castellano, Manuel$$uUniversidad de Zaragoza
000036758 700__ $$aShnyder, S. D.
000036758 700__ $$aVinader, V.
000036758 700__ $$aAfarinkia, K.
000036758 700__ $$0(orcid)0000-0001-5376-4440$$aFernández Ledesma, Luis José$$uUniversidad de Zaragoza
000036758 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa Garrido, Ignacio$$uUniversidad de Zaragoza
000036758 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDepartamento de Anatomía e Histología Humanas$$cHistología
000036758 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDepartamento de Bioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular
000036758 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDepartamento de Ingeniería Mecánica$$cMec. de Medios Contínuos y Teor. de Estructuras
000036758 773__ $$g10, 10 (2015), 0139515 [16pp]$$pPLoS One$$tPLoS One$$x1932-6203
000036758 8564_ $$s4388956$$uhttp://zaguan.unizar.es/record/36758/files/texto_completo.pdf$$yVersión publicada
000036758 8564_ $$s99051$$uhttp://zaguan.unizar.es/record/36758/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000036758 909CO $$ooai:zaguan.unizar.es:36758$$particulos$$pdriver
000036758 951__ $$a2016-06-27-11:20:54
000036758 980__ $$aARTICLE